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Antibody Production and Purification Reagents and Kits
Chemical reagents and kits designed for use in the production and/or purification of specific antibodies; includes substrates, inactivation reagents, standard controls, prepacked columns, purification kits, etc.
Histone extraction kit is designed for the quick isolation of total histone extracts from cells and tissue samples within 1 hour assay time, suitable for mammalian and other species. - Cited in over 110 publications - Isolates histones from as few as 10^5 cells or 1 mg of tissue, with optimal results from >10^6 cells or >10 mg of tissue - High yield – up to 0.4 mg of histone protein per 10^7 cells or 100 mg of tissue - Useful in western blot analysis of histone modifications and post-translational modifications - Not for ChIP; use a chromatin extraction kit for ChIP preparation
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants.